Composite
Pro1

Part:BBa_K4139029:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


Pro1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1728
    Illegal PstI site found at 2028
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal PstI site found at 1728
    Illegal PstI site found at 2028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2012
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1728
    Illegal PstI site found at 2028
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1728
    Illegal PstI site found at 2028
    Illegal NgoMIV site found at 1922
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct begins with the PRO1 protein and is followed by a His-tag to aid in purification.

Source

Plasmodiophora brassicae

References

Feng, J., Hwang, R. U., Hwang, S. F., Strelkov, S. E., Gossen, B. D., Zhou, Q. X., & Peng, G. (2010). Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination. Molecular Plant Pathology, 11(4), 503–512. https://doi.org/10.1111/j.1364-3703.2010.00623.x